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Santa Cruz Biotechnology

TECHNICAL SERVICE GUIDE: Western Blotting

Catalog #:                             Lot #:

PROBLEM AND PREVIOUS EXPERIENCE

1. What is the specific problem you are experiencing?

1. What size bands were expected and what size bands were detected?

1. Was the blot blank or was a dark background or non-specific bands seen?

1. Did this same vial of product work in the past? What were the results?

1. Did other lots of this product work in the past? Which lots? What were the results?

SAMPLES AND CONTROLS

1. From what species （animal） was the sample and what type of sample was used （cell lysate, tissue, purified protein, etc.）? What lysis buffer was used?

1. How much sample was loaded on the gel?

1. If the blocking peptide was used, was it used as a negative control?

1. What positive and/or negative controls were used, and what were the results?

BLOCKING

1. What were the blocking conditions （blocking solution, how long, what temperature. Etc.）?

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